• We derived ESC lines from

  2018-10-22

  

  We derived ESC lines from blastocysts of the double transgenic mouse line Mlc2v-Cre; Rosa26-Yfp, in which Cre and Yfp are specifically knocked into the Mlc2v and Rosa26 gene loci, respectively. Mlc2v-Cre-mediated removal of a stop sequence in the Rosa26-YFP reporter line results in expression of YFP

• Unspecific amplification of gene expression exploits informa

  2018-10-22

  

  Unspecific amplification of gene expression exploits information intrinsic to cells, initiating a self-organizing circuitry, where gene expression cascades propagate and interact, much like the propagation of microscopic fluctuations within a critical iron magnet. Unlike an iron magnet, the circuitr

• In vitro formation and culture

  2018-10-22

  

  In vitro formation and culture of non-adherent spheres from single-cell suspensions has been reported for many tissues, including the salivary glands (Lombaert et al., 2008; Dontu et al., 2003; Reynolds and Weiss, 1996; Weiss et al., 1996). The cultivation of non-adherent spheres in serum-free media

• Unfortunately existing cytogenetic karyotyping methods are l

  2018-10-22

  

  Unfortunately, existing cytogenetic karyotyping methods are labor intensive, requiring at least 15 fixed metaphase preparations, subsequent DAPI staining, and analysis with an epi-fluorescence microscope equipped with specialized digital imaging software. Conventional karyotyping is expensive (∼$400

• We reasoned that we could use engineered tissues

  2018-10-22

  

  We reasoned that we could use engineered tissues as a common test bed for cardiac myocytes from different sources to compare their inherent ability to build themselves and collectively function. Previously, we identified specific subsets of islet-1-expressing, ESC-derived cardiac progenitors that ar

• br Material and methods br Chemotaxis assay br

  2018-10-22

  

  Material and methods Chemotaxis assay Results Discussion In this study we provide evidence that the N-terminal end of CXCL12, a region containing CXCR4 binding and activation domains, is sufficient to induce both migration and proliferation of SVZ-born neuroblasts. Our experimental appro

• br Material and methods br Results br Discussion

  2018-10-22

  

  Material and methods Results Discussion We have demonstrated that deregulation of LMNA in our previously characterized chondrogenic model compromised chondrogenic differentiation through, partially, redox imbalance. Lamin A is a key factor regulating both normal and accelerated aging as wel

• We suggest that one should start with a type of

  2018-10-20

  

  We suggest that one should start with a type of training to increase the SC pool most efficiently. Several human studies reported an increase in SC number of over 80% 5–8days after a single bout of maximal eccentric contraction (Crameri et al., 2004, 2007; O\'Reilly et al., 2008). In contrast, other

• There have been reports showing the linkage between kidney d

  2018-10-20

  

  There have been reports showing the linkage between kidney dysfunction and lipid metabolization (Cignarelli and Lamacchia, 2007; Hall et al., 2003; Kramer, 2006; Maric-Bilkan, 2013; Wickman and Kramer, 2013). Indeed, we found that BMSCs isolated from PNx mice have higher rates of adipogenesis after

• In this report we demonstrated that undifferentiated hESCs

  2018-10-20

  

  In this report, we demonstrated that undifferentiated hESCs expressed moderate levels of HLA-class Ia, no class Ib, and low levels of class II molecules on the cell surface. HLA class Ia processing and presentation are required for effective T cell recognition and impact graft rejection. This findin

• Intestinal stem cells have been identified by various marker

  2018-10-20

  

  Intestinal stem cells have been identified by various markers, such as musashi-1 (Msi1), hairy enhancer of split-1 (Hes1), CD133, leucine-rich repeats and immunoglobulin-like domains protein 1 (Lrig1) (Kayahara et al., 2003; Potten et al., 2003; O\'Brien et al., 2007; Wong et al., 2012). More recent

• In addition we also injected

  2018-10-20

  

  In addition, we also injected the neurospheres into SCID mouse brains. The confocal images showed that the AdSTEP-fragmented neurospheres had abundant PAX6-positive purchase leptomycin b in the cerebral cortex (Fig. 6C) that contained larger NTTR structures, similar to those observed in vitro. These

• These differentiated HLCs retain a strong fetal and immature

  2018-10-20

  

  These differentiated HLCs retain a strong fetal and immature phenotype, as demonstrated by the persistence of alpha-fetoprotein (AFP) and diminished hepatic functions when compared to primary adult hepatocytes (PHHs). Strategies to improve the differentiation protocol are being explored. For example

• br Conflict of interest br Acknowledgements Funding

  2018-10-20

  

  Conflict of interest Acknowledgements Funding is provided by NSF-CMMI 1537745 and Northeast Ohio Medical University to L.N.C. We thank Drs. Mark Stewart (SUNY Downstate) and Ellen Covey (University of Washington) for donating bats to seed the colonies used in this study. Introduction The e

• br Resource table br Resource

  2018-10-20

  

  Resource table Resource details Multiple endocrine neoplasia type 1 (MEN1) (also termed Wermer syndrome) is an autosomal dominant hereditary which is caused by mutations on the tumor suppressor gene MEN1. MEN1 is characterized by tumorigenesis mainly in endocrine organs including parathyroid g

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