Regarding FMR transcription by FXS HESCs we show that FMR

2018-10-20

Regarding FMR1 transcription by FXS HESCs, we show that FMR1 expression in unmethylated FXS HESCs (HEFX, 0% methylation) is indistinguishable from WT HESCs and is significantly reduced in all methylated FXS cell lines. Nevertheless, the relation between the extent of methylation and FMR1 expression

To test whether the direction of the

2018-10-20

To test whether the direction of the genomic strand targeted by the gRNA molecule was a factor in its capacity to recruit dCas9 and activate expression of the endogenous locus, we designed a gRNA molecule (gRNA4) targeting the minus strand of the same region for which gRNA3 was designed (PAM separat

purchase kanamycin To test whether the direction of the

2018-10-20

To test whether the direction of the genomic strand targeted by the gRNA molecule was a factor in its capacity to recruit dCas9 and activate expression of the endogenous locus, we designed a gRNA molecule (gRNA4) targeting the minus strand of the same region for which gRNA3 was designed (PAM separat

br Experimental Procedures br Author

2018-10-20

Experimental Procedures Author Contributions Acknowledgments Introduction Induced pluripotent stem (iPS) cells, generated by introducing defined factors to reprogram terminally differentiated somatic cells, offer enormous potential for the development of autologous or customized cellular

br Results br Discussion Pluripotent stem cells

2018-10-20

Results Discussion Pluripotent stem 4-ethylphenyl sulfate offer unprecedented potential for regenerative medicine as they can be differentiated by a specific sequence of signals to become any cell type of the body. At the same time, any cell product generated from them confers a substantial r

Surprisingly few studies on human fetal livers have reported

2018-10-20

Surprisingly, few studies on human fetal livers have reported that the hematopoietic progenitor markers CD34, CD117, CD90, CD133, and CD44 are also expressed on a subset of human hepatoblasts and/or precursors of hepatoblasts depending on the embryonic stage examined (Table S1). Most of the EpCAM+ h

However in these models human hematopoietic

2018-10-20

However, in these models, human hematopoietic reconstitution is usually B-lymphoid dominant, and engraftment of human myeloid, erythroid, and megakaryocyte lineages is limited (Doulatov et al., 2012; Ito et al., 2012; Rongvaux et al., 2013; Yamauchi et al., 2013). This skewed reconstitution could be

Hello world!

2018-07-29